Suitable materials are essential for success when growing mushrooms in jars. So let’s start learning how to grow mushrooms in bottles and jars!
Mushrooms are a culinary delicacy and have recently become famous for cultivating at home. Growing mushrooms in jars is an efficient and cost-effective way to enjoy this versatile food source.
From adding flavor to dishes like risotto or pizza or cooking into sauces for that Earthy umami taste, there’s no limit to the dishes you can introduce them into!
So let’s start growing mushrooms in bottles process- with some simple steps and supplies, you can pick your fresh mushrooms right from your windowsill set-up!
Materials Needed For Growing Mushrooms In bottles
When growing mushrooms in jars, suitable materials are essential for success. Here are some of the necessary items you will need to get started:

1. Jars or Bottles
Glass jars and bottles are ideal for growing mushrooms. You can use quart-sized jars, but half-pint jars are recommended for beginners.
Ensure the jars are free of cracks, chips, or other damage.
2. Substrate
This is the material that the mushroom spores or spawn will grow in.
Popular substrate choices include vermiculite, brown rice flour, and perlite. You can purchase a pre-made substrate or make your own.
3. Spores or Spawn
Spores are the reproductive cells of mushrooms, while spawn is a pre-grown mycelium that has been inoculated with spores. You can purchase spores or spawn online or from a local supplier.
4. Pressure Cooker
Sterilization is vital when growing mushrooms in jars. A pressure cooker is the best way to sterilize the substrate and kill any unwanted bacteria or fungi.
5. Gloves and Mask
You should wear gloves and a mask when handling the substrate and jars to avoid contamination.
6. Drill and Tin Foil
It would be best if you drilled holes in the jar lids to allow air exchange. Cover the holes with tin foil until it’s time for the mushrooms to fruit.
7. Spray Bottle
Maintaining proper humidity levels is essential when growing mushrooms. A spray bottle can be used to mist the jars and keep the substrate moist.
8. Grow Light
While it’s not entirely necessary, a grow light can enhance the growth and consistency of fruiting of your mushrooms.
It helps provide additional light and heat that mimics natural sunlight, encouraging healthy growth and robust yields.
It is beneficial in areas with minimal sunlight and for those looking to achieve faster and more bountiful yields.
Also read- 11 Types Of Edible Mushrooms Identification In Backyard
Preparing the Jars For Mushrooms

Preparing the jars is an essential step in growing mushrooms in jars, and it’s crucial to do it right to ensure the mushrooms grow in a healthy environment.
Below are the steps to take when preparing jars for mushroom cultivation:
1. Cleaning the Jars
It’s essential to clean jars thoroughly before filling them with the substrate.
Any bacteria or debris left inside the jars can contaminate the substrate and prevent the mushrooms from growing.
You can wash the jars with warm soapy water and rinse with hot water or sterilize them by boiling them in water for 10-15 minutes.
2. Preparing the Lid
To create a suitable environment for your mushrooms to grow, you must modify the lid of your jar. The objective is to create a sterile environment that allows for adequate airflow.
You can achieve this by drilling small holes in the lid and covering them with filter disks or synthetic filter patches.
The filter disks or patches permit air to circulate while preventing contaminants from entering the jars.
3. Filling the Jars with Substrate
After ensuring your jars are clean and sterile, the next step is to fill them with the substrate. The substrate provides the necessary nutrients for the mushroom mycelium to thrive.
You can use a variety of substrates, such as brown rice flour, vermiculite, and water, depending on the type of mushroom you’re growing.
Begin by adding a substrate layer to the bottom of the jar and then firmly press it down with a spoon.
Repeat this process until you’ve filled the jar, leaving an inch of space at the top. You can inoculate your jars and begin incubating with the adequately filled substrate.
Inoculating the Jars
Inoculating the jars is a crucial step in growing mushrooms. It involves introducing spores or spawning into the jars to initiate growth.
Here’s a detailed guide on how to inoculate your jars properly:
1. Obtaining Spores or Spawn
The first step is to obtain spores or spawn from a reputable source.
Spores are tiny reproductive structures of mushrooms that are used to create mycelium, the vegetative part of the mushroom.
On the other hand, spawn is the mycelium that has already colonized a substrate. You can purchase either spores or spawn from a reliable supplier or harvest them from a mature mushroom.
2. Sterilizing the Spores or Spawn
Before inoculating those jars, it’s crucial to sterilize the spores or spawn.
Lucky for you, there are a few different ways to ensure the job is done right. You can try using a trusty pressure cooker, nuking those spores in the microwave, or even opting for a chemical sterilizer.
No matter which method you choose, always follow the instructions provided by your supplier or consult a guide to keep your fungi thriving!
3. Injecting the Spores or Spawn into the Jars
Once you’ve correctly sterilized your spores or spawn, it’s time to get them into your jars. Ensure your injection site is spotlessly clean and sterilized to ensure optimal results.
And luckily for you, the most common method for doing so is through a self-healing injection port.
This easy-to-install portal allows for swift, sanitary access to the inside of the jar.
Alternatively, for those without the injection port, a sterile syringe can inject spores or spawn through a small hole in the jar’s lid.
4. Sealing the Jars
After inoculation, the jars must be sealed to prevent contamination from the outside environment.
Use the lid provided with the jar or a custom lid with a self-healing injection port. Tighten the cap securely to prevent air or contaminants from entering the jar.
5. Incubating the Jars
Place the inoculated jars in a warm, dark, and humid place for the spores or spawn to grow into mycelium.
Incubating the jars is a crucial step in growing mushrooms in jars. You’ll create optimal conditions for your mushrooms to thrive during this stage.
6. Creating a Favorable Environment
To begin, it’s essential to create an environment that is conducive to mushroom growth. This involves maintaining a steady temperature and humidity level.
Ideally, the temperature should be between 70 and 75 degrees Fahrenheit and the humidity level should be between 80 and 95 percent.
One way to maintain these conditions is to place the jars in a terrarium or grow box.
You can create a simple terrarium by covering the jars with a clear plastic bag and misting the inside of the bag regularly to maintain the desired humidity level.
Alternatively, you can purchase a ready-made grow box, which typically comes equipped with a humidity and temperature control system.
7. Optimal Temperature and Humidity Levels
Keeping the temperature and humidity levels consistent throughout the incubation stage is essential.
Drastic changes in temperature or humidity can stunt or kill the mycelium, the network of fungi that grows inside the substrate.
8. Signs of Healthy Growth
After a few days of incubation, you should see signs of healthy growth. The mycelium will begin to spread throughout the substrate, forming a web-like network.
If the mycelium appears fuzzy or has a strange odor, this could indicate contamination. If this occurs, discarding the jar and starting over is best.
Fruiting the Bottles
Once the mycelium has fully colonized the substrate in the jars, it is time to initiate the fruiting process.
This stage is also referred to as the “pinning” stage, as it involves the formation of small pins or knots on the surface of the substrate.
These pins will eventually develop into mature mushrooms.
1. Initiating the Fruiting Process
The fruiting process is an important stage in growing mushrooms in jars. It is initiated by exposing the jars to fresh air and increasing humidity.
Placing the jars in a fruiting chamber is recommended to create an optimal environment.
A fruiting chamber can be as simple as a plastic container with ventilation holes.
It is essential to keep the temperature between 60-75°F and maintain a humidity level of around 90% for the best results.
2. Caring for the Growing Mushrooms
As the pins start forming on the surface of the substrate, it is essential to monitor the humidity level and ensure proper ventilation within the fruiting chamber.
Mushrooms thrive in high humidity levels, but excess moisture can lead to bacterial contamination.
Regular misting of the inside of the fruiting chamber with water is essential to maintain the required humidity levels.
3. Harvesting the Mushrooms
Mushrooms can be harvested once they have reached maturity.
The harvest timing will depend on the species of mushroom being grown, but generally, mushrooms are ready to be harvested when the cap has fully opened and the veil underneath the lid has started to break.
To harvest the mushrooms, twist and pull them gently from the substrate, careful not to damage the surrounding pins.
Growing Mushrooms In bottles FAQs
Growing mushrooms in jars can be a fascinating and fulfilling experience, but it can also be challenging for beginners.
You might have questions or concerns if you’re new to the process. To help you grow healthy and tasty mushrooms, we’ve put together some common questions and answers:
Q: How Do I Know If My Jars Are Fully Colonized With Mycelium?
Check for white, web-like mycelium throughout the substrate to confirm that your jars are colonized.
When the substrate is fully covered in mycelium, it is colonized.
Typically, colonizing takes about 2-4 weeks, depending on the mushroom species and incubation conditions.
Q: Can I Reuse Jars That Have Already Been Used For Mushroom Cultivation?
Yes, you can reuse jars for mushroom cultivation, but it is crucial to sterilize them adequately before using them again.
First, wash the jars with soap and water, then sterilize them in a pressure cooker or autoclave to ensure they are contamination-free.
Q: Can I Grow Different Species Of Mushrooms In The Same Jar?
Growing mushroom species in a different jar is not recommended because they may compete for resources and space, resulting in poor growth.
Stick to one species per jar for optimal results.
The Bottom Line
Growing your mushrooms can be a rewarding and fun experience. However, it does have its challenges.
Take the time to understand the environment and basic requirements at each growth stage, and you will eventually become successful in growing your mushrooms in jars.
With all these tips and careful preparation, we hope you feel confident enough to grow delectable mushrooms in jars yourself!

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